I have one question, "how to build 16S rRNA similarity matrix?".
I am trying to reclassify a family of bacteria, but inorder to do that I need to blastn multiple 16S rRNA sequences againts each other to build matrix, about 400 sequences. I use blastn, but the result did not give me any matrix. Is there any other method to do this? or I am missing something here.
Thank you for taking time to reply,