I'm analyzing whole exam sequencing data, and using Picard to perform some QC on my aligned / sorted / duplicated removed .bam files. When running
picard CollectSequencingArtifactMetrics, I receive the following error:
Exception in thread "main" picard.PicardException: Record contains library that is missing from header: UnknownLibrary
WES data processing / analysis is not my main area of expertise at all, and I'm not super familiar with the .sam / .bam formats. Does anyone have any idea what could be causing this? I've run other
picard functions, such as
MarkDuplicates without errors, so I'm pretty confused.