Question: How To Use Blast+ Remote Option
2
gravatar for snakesgun
8.1 years ago by
snakesgun140
snakesgun140 wrote:

Hello all,

I try to use ncbi remote database to make a blast search, but it failed. There is a warning message I don't understand. How can I use this option?

The warning message is

Please make sure your firewall does not block ports 4444-4544 bound for NCBI(130.14.29.112/165.112.7.12)

my command line is

$ blastn -db nr -query sequence.fasta -remote -out result.bls

Thanks~!

• 10k views
ADD COMMENTlink modified 8.1 years ago by bow790 • written 8.1 years ago by snakesgun140

how we can search proteins query sequences with EST database remotely for BLASTp

Thanks

ADD REPLYlink written 4.9 years ago by Manoj40

How to BLAST proteins query remotely with species specific such as some selected plants

 

Thanks

ADD REPLYlink written 4.9 years ago by Manoj40
2
gravatar for bow
8.1 years ago by
bow790
Netherlands
bow790 wrote:

It says that port 4444-4544 are not accessible. Ports can be considered as channels through which your computer accepts data. In this case, the blast program needs to use port number 4444 and 4544.

I guess this error could be caused by two things: 1) some other software is using those ports or 2) it's blocked in your network. If you're on a campus/office network, you can perhaps ask the IT guy see which one it is and to try fix it.

ADD COMMENTlink written 8.1 years ago by bow790
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1185 users visited in the last hour