Entering edit mode
3.9 years ago
profnf11295
•
0
Hello,,
I'm working on a project with ORFs of 10 bacterial genomes, i extracted my ORFs using emboss getorf tool then i looked for an overlap between ORFs and CDS of those bacterial genomes, i've found almost 65% of ORFs overlap with CDS at either start codon or end codon and scientifically in prokaryotes it's abnormal .. so is there anyone have any possible explanation ? and what is exactly the difference between emboss getorf and ncbi getorf ?
Can you clarify if these are new genomes or genomes that have been newly sequenced? If latter, you may want to use a program like
prokka(LINK) instead of simple ORF programs.Also, please try to support us with the command parameters that you used. Make sure that you selected Bacteria when you run the program.