ORFs finding tools
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18 months ago

Hello,,

I'm working on a project with ORFs of 10 bacterial genomes, i extracted my ORFs using emboss getorf tool then i looked for an overlap between ORFs and CDS of those bacterial genomes, i've found almost 65% of ORFs overlap with CDS at either start codon or end codon and scientifically in prokaryotes it's abnormal .. so is there anyone have any possible explanation ? and what is exactly the difference between emboss getorf and ncbi getorf ?

ORF CDS genome getorf findorf • 611 views
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Can you clarify if these are new genomes or genomes that have been newly sequenced? If latter, you may want to use a program like prokka (LINK) instead of simple ORF programs.

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Also, please try to support us with the command parameters that you used. Make sure that you selected Bacteria when you run the program.