I do not understand a lot of things in bioinformatics, so the answer to my question is probably over there, sorry if I did not realize it.
I want to know if some genes are expressed in an organ, and I already have some transcriptome SRA numbers but I do not know how to do the searching. I have read about the SRA toolkit but I do not know to program, and about the SRA database in BLAST. So my questions are
1) ¿Is there another way to search if a gene is represented in the given transcriptome other than SRA toolkit?
2) I understand that SRA contains the reads given by the sequencer. So If I do a BLAST with the SRA database
a. What should I introduce the gene or the RNA?
b. Will it give only reads (nothing annotated, any protein name)?
c. If yes, how can I say those reads are from some specific gene?
Thank you very much for your time, Mónica.