Question: Does DESeq2 can do hypothesis testing between multiple treatment groups or the tests just done between the genes ?
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gravatar for shome
14 days ago by
shome0
shome0 wrote:

I have five datasets from the samples where I am trying to compare the miRNA abundances between the control and different treatments. Is it possible to use DESeq 2 to do hypothesis testing to suggest there is a significant difference between the control and treatments or not ?

rna-seq • 67 views
ADD COMMENTlink modified 14 days ago • written 14 days ago by shome0
1

Have you read the DESeq2 vignette? While the logFC is determined by genes, the p-value depends on the group size, so DESeq2 compares groups, not just genes.

ADD REPLYlink written 14 days ago by RamRS27k
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