I am a bioinformatics student and completely new to NGS analysis (and also to biological reasoning). I would like to understand what is all about the variant allele frequency (VAF).
Why is it important to know whether the VAF is highly enough? What VAF can be considered highly enough?
Is there a specific cutoff for VAF that can be used to determine whether a variant is clonal or subclonal, assuming a 90% tumor purity?
I tried googling it but could not figure out a satisfying answer. Appreciate your effort.