Any other way to validate RNA-seq data if qPCR tests is not available?

Question

[shome]

I have some sequencing data which has been computationally analyzed for GO, and Differential expression analysis. Unfortunately,qPCR wasn't done for the samples. Is there any way/method we can confirm our findings are validated?

[dsull]

Relevant twitter thread:

https://twitter.com/andy_utoronto/status/1266144011259195394

Answer 1

[Kevin Blighe]

I would try to replicate the findings in publicly-available datasets. You will likely find some data on Gene Expression Omnibus (GEO).

Kevin