Off topic:Immacantation_tigger library function inferGenotype gives following error Error in strsplit(., ",") : non-character argument**

Question

[B- for bioinformatics]

Hi "trigger"'s function inferGenotype shows error in strsplit (See below).

Rstudio_Rnotebook file

```{r}
library(tigger)
library(alakazam)
library(dplyr)

```




```{r}

lung0_2 <- readChangeoDb("Z:/light_IGLK_lung0_2_parse-select.tab")

Notes: Reading mouse immunoglobulin light chain V and J region genes fasta file
ighv <-readIgFasta("Z:/imgt_mouse_IGKJ_V.fasta")

```

```{r}
colnames(lung0_2)


 [1] "SEQUENCE_ID"        "SEQUENCE_INPUT"     "FUNCTIONAL"         "IN_FRAME"           "STOP"               "MUTATED_INVARIANT"  "INDELS"             "LOCUS"              "V_CALL"             "D_CALL"            
[11] "J_CALL"             "SEQUENCE_VDJ"       "SEQUENCE_IMGT"      "V_SEQ_START"        "V_SEQ_LENGTH"       "V_GERM_START_VDJ"   "V_GERM_LENGTH_VDJ"  "V_GERM_START_IMGT"  "V_GERM_LENGTH_IMGT" "NP1_LENGTH"        
[21] "D_SEQ_START"        "D_SEQ_LENGTH"       "D_GERM_START"       "D_GERM_LENGTH"      "NP2_LENGTH"         "J_SEQ_START"        "J_SEQ_LENGTH"       "J_GERM_START"       "J_GERM_LENGTH"      "JUNCTION"          
[31] "JUNCTION_LENGTH"    "GERMLINE_IMGT"      "V_SCORE"            "V_IDENTITY"         "V_EVALUE"           "V_CIGAR"            "D_SCORE"            "D_IDENTITY"         "D_EVALUE"           "D_CIGAR"           
[41] "J_SCORE"            "J_IDENTITY"         "J_EVALUE"           "J_CIGAR"            "FWR1_IMGT"          "FWR2_IMGT"          "FWR3_IMGT"          "FWR4_IMGT"          "CDR1_IMGT"          "CDR2_IMGT"         
[51] "CDR3_IMGT"          "CELL"               "C_CALL"             "CONSCOUNT"          "UMICOUNT"           "V_CALL_10X"         "D_CALL_10X"         "J_CALL_10X"         "JUNCTION_10X"       "JUNCTION_10X_AA"




```{r}

nv <- findNovelAlleles(lung0_2, ighv, v_call = "V_CALL", j_call = "J_CALL", seq = "SEQUENCE_IMGT", junction = "JUNCTION", junction_length = "JUNCTION_LENGTH", germline_min = 10, min_seqs = 10, auto_mutrange = TRUE, mut_range = 1:10, pos_range = 1:312, y_intercept = 0.125, alpha = 0.05, j_max = 0.15, min_frac = 0.75, nproc = 1)

selectNovel(nv) %>% select(germline_call, polymorphism_call)


```


```{r}
gt <- inferGenotype(lung0_2, germline_db=ighv, novel=nv)
```
**Traceback**
**Error in strsplit(., ",") : non-character argument**
10.strsplit(., ",")
9.function_list[[i]](value)
8.reduce(value, `_function_list`)
7.`_fseq`(`_lhs`)
6.eval(quote(`_fseq`(`_lhs`)), env, env)
5.eval(quote(`_fseq`(`_lhs`)), env, env)
4.withVisible(eval(quote(`_fseq`(`_lhs`)), env, env))
3.allele_calls %>% strsplit(",") %>% unlist %>% setdiff(names(germline_db))
2.findUnmutatedCalls(allele_calls, as.character(data[[seq]]), germline_db)
1.inferGenotype(lung0_2, germline_db = ighv, novel = nv)