Hi everyone, I have a dataset with count reads of miRNA-Seq downloaded from TCGA: on the rows I have the miRNA names and on the columns the uuid barcodes(sample names).
When I launch the
vst function it gets me this error:
dd = DESeqDataSetFromMatrix(d, coldata, design= ~class) vst = vst(dd, blind = FALSE) Error in vst(dd, blind = FALSE) : less than 'nsub' rows with mean normalized count > 5, it is recommended to use varianceStabilizingTransformation directly
This is the first attempt with this package, any suggestion? Thank you so much