Convert .gbff (gene bank flat format) to gff3, gtf
1
0
Entering edit mode
3.7 years ago
alex • 0

As an example, I'm working with a reference genome for pineapple and need a GTF and GFF file. This is the assembly ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/902/162/155/GCA_902162155.2_F153.v0709/

All that's available is a .gbff file. I'm looking for a way to convert this into a gff3 and gtf file.

I am able to convert the gbff into a gff3 using bp_genbank2gff3

Using genome tools, I can validate this gff3 by running

gt gff3validator my.gff

and it tells me it's valid. But when i try and convert this gff to a gtf using either genome tools

(gt gff3_to_gtf my.gff)

or gffread

(gffread my.gff3 -T -o my.gtf)

it generates an empty file.

Am i going about the the right way?
Any help is appreciated

genome gtf gff gbff genebank • 12k views
ADD COMMENT
0
Entering edit mode

If you are able to use a different assembly then there is a GFF file available.

ADD REPLY
3
Entering edit mode
3.7 years ago
Juke34 8.5k

It is not annotated, there is no gene model in the gbff file.

ADD COMMENT
0
Entering edit mode

So essentially this is not possible? Is there any other way to generate a gtf in this case?

ADD REPLY
1
Entering edit mode

You can perform your own annotation, but it can be quite tough

ADD REPLY
0
Entering edit mode

Understood. Thank you very much for your answer!

ADD REPLY

Login before adding your answer.

Traffic: 1806 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6