What does the (norm) suffix mean in the miRDeep2 quantifier.pl output file?

Question

[Nicolai Skovbjerg Arildsen]

Hello everyone!

I've run the miRDeep2 quantifier.pl pipeline on 12 samples, and have gotten a nice results table. However, it looks like this:

  miRNA read_count  precursor   total   s01 s02 s01(norm)   s02(norm)
  hsa-let-7a-5p 60215100    hsa-let-7a-1    60215100    3933700 2594200 5499125 835850

So I'm wondering what the s01(norm) and s02(norm) is? Normalized numbers? And if so, how are these normalized and with regards to what?

Answer 1

[JC]

yes,the suffix means normalization, the code indicates it is a tpm:

## Author: SM
## Date: 04/04/2019
## read normalization is now 1e6 * mature-reads/all_mature_reads (So reads per million mapped miRNA reads)
## precursors with 0 read mappings cryptic warning messages to stderr removed