News:DNA Methylation Data Analysis Workshop - 2-5 November 2020 in Berlin
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DNA Methylation Data Analysis

How to use bisulfite-treated sequencing to study DNA methylation

Link: DNA Methylation Workshop

When? 2-5 November 2020

Where? PC-College, Stresemannstraße 78, 10963 Berlin, Germany

Scope and Topics

The purpose of this workshop is to get a deeper understanding of the use of bisulfite-treated DNA in order to analyze the epigenetic layer of DNA methylation. Advantages and disadvantages of the so-called 'bisulfite sequencing' and its implications on data analyses will be covered. The participants will be trained to understand bisulfite-treated NGS data, to detect potential problems/errors and finally to implement their own pipelines. After this course they will be able to analyze DNA methylation and create ready-to-publish graphics.

By the end of this workshop the participants will:

  • be familiar with the sequencing method of Illumina
  • understand how bisulfite sequencing works
  • be aware of the mapping problem of bisulfite-treated data
  • understand how bisulfite-treated reads are mapped to a reference genome
  • be familiar with common data formats and standards
  • know relevant tools for data processing
  • automate tasks with shell scripting to create reusable data pipelines
  • perform basic analyses (call methylated regions, perform basic downstream analyses)
  • plot and visualize results (ready-to-publish)
  • be able to reuse all analyses

Target Audience

  • biologists or data analysts with no or little experience in analyzing bisulfite sequencing data

Included in the Course

  • Course materials
  • Catering
  • Conference Dinner


  • Dr. Helene Kretzmer (Max Planck Institute for Molecular Genetics) is working on DNA methylation analyses using high-throughput sequencing since 2011. She is responsible for the bioinformatic analysis of MMML-Seq study of the International Cancer Genome Consortium (ICGC).
  • Dr. Christian Otto (ecSeq Bioinformatics GmbH) is one of the developers of the bisulfite read mapping tool segemehl and is an expert on implementing efficient algorithms for HTS data analyses.

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