News: DNA Methylation Data Analysis Workshop - 2-5 November 2020 in Berlin
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David Langenberger9.5k wrote:

DNA Methylation Data Analysis

How to use bisulfite-treated sequencing to study DNA methylation

Link: DNA Methylation Workshop

When? 2-5 November 2020

Where? PC-College, Stresemannstraße 78, 10963 Berlin, Germany

Scope and Topics

The purpose of this workshop is to get a deeper understanding of the use of bisulfite-treated DNA in order to analyze the epigenetic layer of DNA methylation. Advantages and disadvantages of the so-called 'bisulfite sequencing' and its implications on data analyses will be covered. The participants will be trained to understand bisulfite-treated NGS data, to detect potential problems/errors and finally to implement their own pipelines. After this course they will be able to analyze DNA methylation and create ready-to-publish graphics.

By the end of this workshop the participants will:

  • be familiar with the sequencing method of Illumina
  • understand how bisulfite sequencing works
  • be aware of the mapping problem of bisulfite-treated data
  • understand how bisulfite-treated reads are mapped to a reference genome
  • be familiar with common data formats and standards
  • know relevant tools for data processing
  • automate tasks with shell scripting to create reusable data pipelines
  • perform basic analyses (call methylated regions, perform basic downstream analyses)
  • plot and visualize results (ready-to-publish)
  • be able to reuse all analyses

Target Audience

  • biologists or data analysts with no or little experience in analyzing bisulfite sequencing data

Included in the Course

  • Course materials
  • Catering
  • Conference Dinner

Trainers

  • Dr. Helene Kretzmer (Max Planck Institute for Molecular Genetics) is working on DNA methylation analyses using high-throughput sequencing since 2011. She is responsible for the bioinformatic analysis of MMML-Seq study of the International Cancer Genome Consortium (ICGC).
  • Dr. Christian Otto (ecSeq Bioinformatics GmbH) is one of the developers of the bisulfite read mapping tool segemehl and is an expert on implementing efficient algorithms for HTS data analyses.

Apply Now

      

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