I'm sorry if this is answered somewhere else, If it is please direct me to it. I cant seem to find much information.
I'm wondering how DNA fragment clusters dont get all mushed together on a flow cell when sequencing after bridge amplification.
It is my understanding that during RNA-Seq, RNA is converted to DNA, then the DNA is fragmented. The resulting fragments are layed out on a flow cell, amplified by bridge amplification to get stronger light detection, and then sequenced.
So my confusion stems from this thought: If DNA fragments are dispersed randomly throughout a flow cell, and then amplified by bridge amplification, what is preventing the clusters from merging? I made a picture (see link below) to better describe my question.
Pictorial depiction of my confusion
I guess my question could also be reworded as "What prevents DNA fragments from amplifying too close to each other in a flow cell?"
Thank you in advance!
Excellent, Thank you very much. This answers my non-bioinformatics question
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