Entering edit mode
3.6 years ago
lokdeep17
•
0
I searched a gene of interest in a species using SRA BLAST web interface. From graphic summary I know the gene is fully covered with good read support. Now I want to generate a consensus sequence from these reads but I am finding it hard to do so. I will be very very thankful if someone can teach me to do so. The output of aligned reads is in FASTA format. I know it is a naive question to ask, but I am really stuck at this.
If you are able to download the aligned fasta reads you should be able to generate a consensus via MEGA (if you need a graphical interface) or via any multiple sequence alignment programs.