Blastx against nr for plants

Question

[m.s_tabatabaei]

Hi every one,

I run the Blastx aginst nr using this commend line "

blastx -db blastdb_Nr/Blast_Nr -query Query
 -taxidlist 33090.txids -evalue 1.0e-5 -num_threads 23 -word_size 3 -max_target_seqs 1 -max_hsps 1 -outfmt 6 -out res_blastx

I got this error that " Taxonomy ID(s) not found. This could be because the ID(s) provided are not at or below the species level ". Actually, I got the Taxonomy ID(s) using " get_species_taxids.sh -t 33090 > 33090.txids ".

I am so thankful if you could help me to solve this problem. Mina

[Fatima]

Hi Is your -db correct?

Have you tried to blastx against a "species-level taxonomic node" to make sure your -db is set correctly?

https://www.ncbi.nlm.nih.gov/books/NBK546209/

This post might also help: Restricting database by taxa in local blast searches