I am performing quality control on some reads that have good FastQC metrics apart from duplication levels and per base sequence content. Is it necessary to trim these reads before alignment and downstream DE analysis, variant calling etc? I have read conflicting advice about trimming before RNAseq analysis and not sure what to do.
Question: Is trimming necessary for RNAseq?
9 weeks ago by
Nish314 • 0
Nish314 • 0 wrote:
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