Question: RNA seq analysis ( pre-processing)
gravatar for najioumayma
2 days ago by
najioumayma0 wrote:

Hi everyone, i really need your help, I am a beginner in R and also bioinformatics, my problem is that I want to do a differential analysis in R, I have a dataset from TCGA with 27 genes from 1083 patients , i want to see the expression of gene in two conditions when i was searching about that I find that i have to start by a pre-treatment analysis first but when i found some scripts i noticed that my dataset is very different from others and i don't know how to start. Please can anyone help me to find a solution? thank you in advance

rna-seq R • 68 views
ADD COMMENTlink written 2 days ago by najioumayma0

Well, nobody can help you because we can neither see the format in which your data is stored. To maximise the possibilities of receiving help, you need to provide a minimal reproducible example [of data]. You should also show some lines of code that you have already tried so that we are sure that we are not just doing your work for you. You should also explain from where, exactly, you got your data. Finally, which "scripts" did you find? Thanks.

ADD REPLYlink modified 2 days ago • written 2 days ago by Kevin Blighe67k

With only 27 genes you will have a hard time normalizing data properly, but as Kevin says, please add details and clarity.

ADD REPLYlink written 2 days ago by ATpoint41k
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