Marine Microorganisms Mining
1
0
Entering edit mode
24 months ago
August • 0

How did people get marine microorganisms' gene sequence? Is there a database that contains a lot of sequences? How do the experiments proceed?

marine genome BGCs Biosynthetic Cluster • 462 views
ADD COMMENT
0
Entering edit mode

Get water -> extract DNA -> sequence genomes.

You might want to look at initiatives like TARA:

http://ocean-microbiome.embl.de/companion.html

https://www.ebi.ac.uk/services/tara-oceans-data

Of course NCBI is full of marine microbial data, though it is not necessarily curated as such.

There are lots of resources out there for these types of data - this is by no means an exhaustive list, and TARA probably isn't even the de facto data source, though it is one of the biggest studies AFAIK. This is not really my area though.

ADD REPLY
0
Entering edit mode

Marine prokaryotes or eukaryotes? Most prokaryotes are sequenced "directly" via metagenomics approaches. Eukaryotes (mostly) depending on their size can be isolated by hand, or under a microscope, and sacrificed thereafter to extract nucleic acids for sequencing. (I do believe "eukaryotic" metagenomics exists as well.)

ADD REPLY
0
Entering edit mode
24 months ago
Fatima ▴ 1000

Marine Sediment Metagenome

You can use a gene predictor (e.g. FragGeneScan) to predict the genes ....

ADD COMMENT

Login before adding your answer.

Traffic: 1852 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6