I am a computer scientist who needs to learn some biology. I'm finding this tough as many of the terms seem to refer to the very similar things which I don't have enough knowledge to resolve.
What is the difference between DNA resequencing and genotyping? I have read about the use of DNA chips to resequence and individual's DNA to find out where their DNA differs from the reference genome. But i have also heard the term genotyping used to find out the genotype of an individual and hence see how their DNA sequences differs from the refernce genome.
DNA resequencing and genotyping seem like the same thing to me. The best I can think of is that DNA resequencing is one method to genotype something and there were other methods before chips came along. I can see from wikipedia that there are many different methods of genotyping
BlockquoteGenotyping is the process of elucidating the genotype of an individual with a biological assay. Also known as a genotypic assay, techniques include PCR, DNA fragment analysis, allele specific oligonucleotide (ASO) probes, DNA sequencing, and nucleic acid hybridization to DNA microarrays or beads. Several common genotyping techniques include restriction fragment length polymorphism (RFLP), terminal restriction fragment length polymorphism (t-RFLP), amplified fragment length polymorphism (AFLP), and multiplex ligation-dependent probe amplification (MLPA).
Are all of these tehcniques used with equal prevalence is one of them industry standard or the preferred one? I don't really want to get confused by reading about a whole load of tehniques that are soon to be obsolete.
thanks for your time