How To Retrieve More Than One Protein Sequence In Fasta Files For Knows Accession Id ?

Question

[Kits]

Hi , I want to collect mutiple protien sequences of know accesion ID in fasta format in single file via BioMart MARTVIEW ?

Sample list :-Q7VQX0,P24226,Q8YF59,Q9ZHE6,P62458,Q3SW26

[Neilfws]

And you've tried to do so, but have some problem?

[Kits]

Yes, I did some work. 1.Select database = UniProt (uk) 2.check on accession and sequence length in "Filters" 3.paste my list in the box 4.In "Attributes" select the "Protein name" 5.Click "Results" However, I had retrieved Accession,Entry name and Protein name but not protein sequences.

[Neilfws]

Well, you were very close. Under "Attributes", you need to select the "Sequences" option. You are then presented with further options as to what type of sequence and what to put in the Fasta header.

Answer 1

[Elisabeth Gasteiger]

If you are not limited to BioMart, you can use the "Retrieve" tab on the UniProt web site, http://www.uniprot.org/batch/ to submit your identifiers.

[Kits]

Thank you Elisabeth , its work more easier then BioMart.

[Neilfws]

The UniProt site is very good and since you have UniProt IDs, a good solution for you. However, I would recommend getting to know BioMart, as it is a very powerful and useful tool. Plenty of tutorials exist including video (just search Google for "biomart tutorial").