Question: Bwa/Samtools Soft-Clipping To Retrieve Back Whole Read
2
gravatar for 2184687-1231-83-
8.1 years ago by
2184687-1231-83-4.9k wrote:

Hi,

(I've been googling for this and couldn't find the answer)

Am I right in thinking that the default bwa/samtools instructions in the website will hard-clip the reads in the SAM/BAM files?

In the usual pipeline for single fragment reads: bwa aln ... bwa samse ... samtools view ... samtools sort ... samtools index ...

How can I end up with a final SAM/BAM file that contains the whole of the read/qualscore even if it's not fully matched?

Cheers

samtools bwa • 3.3k views
ADD COMMENTlink written 8.1 years ago by 2184687-1231-83-4.9k
3

No, it is soft clip.

ADD REPLYlink written 8.1 years ago by lh331k
3
gravatar for Ketil
7.9 years ago by
Ketil3.9k
Germany
Ketil3.9k wrote:

I've been using bwa, and I see soft-clipped reads in the results. I doubt that samtools will change this. As far as I can tell, the BAM files also contain the whole reads.

ADD COMMENTlink written 7.9 years ago by Ketil3.9k
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