**680**wrote:

Hi, while i run cuffdiff I got an error like this:

```
cuffdiff -p 8 -o /s1_s2 -b /bowtie2index/hg19.fa -u /all_merged/merged.gtf /s1.bam /s2.bam
[20:01:09] Loading reference annotation and sequence.
Error (GFaSeqGet): end coordinate (108996652) cannot be larger than sequence length 107349540
Error (GFaSeqGet): end coordinate (108996652) cannot be larger than sequence length 107349540
Error (GFaSeqGet): end coordinate (108996652) cannot be larger than sequence length 107349540
Error (GFaSeqGet): end coordinate (108996652) cannot be larger than sequence length 107349540
Error (GFaSeqGet): end coordinate (108996652) cannot be larger than sequence length 107349540
Error (GFaSeqGet): end coordinate (108996652) cannot be larger than sequence length 107349540
Error (GFaSeqGet): end coordinate (108996652) cannot be larger than sequence length 107349540
Error (GFaSeqGet): end coordinate (108996652) cannot be larger than sequence length 107349540
```

Anyone knows this? Thanks!!

Are you running on a 32-bit system?

2.3krunning on unix cluster. Do you mean that is because of cpu or memory exhausted?

680Then most likely not. I was just wondering if the allocation was not possible due to memory restrictions of a 32-bit architecture.

2.3k