Hi, I try to extract sequences from a fastq file using the "subseq" in seqtk. But the extract file contains only the 1st sequence but no others. I am wondering whether my name.lst file does not fit with what seqtk needs. I have names of each sequence without other symbols each line in the name.lst. But the fastq file starts each sequence name with a @. Should I add @ in front of each sequence name? Or what other problem it can be?
Any suggestion is welcome. Thanks,