Question: How To Know Which Is The Start Codon And Stop Codon For A Gene Sequence?
1
gravatar for Elena
8.7 years ago by
Elena240
Elena240 wrote:

Hi Friends,

I have a sequence and want to know the start site and the stop site in that particular gene. for eg: NM_000465 is my gene sequence and want to know the start site and the stop site of the sequence so that i can translate the sequence to a proper protein sequence.

genome sequence position • 29k views
ADD COMMENTlink modified 8.7 years ago by Neilfws48k • written 8.7 years ago by Elena240
4

In the case of completely annotated sequences, search NCBI Nucleotide for your sequence and check the link for the protein record. If it was not available, you could use tools like ORF Finder: http://www.ncbi.nlm.nih.gov/projects/gorf/

ADD REPLYlink written 8.7 years ago by Michael Schubert6.9k
1

And don't use ALL CAPS!

ADD REPLYlink written 8.7 years ago by Michael Dondrup47k

@Schubert: Thanku & @Dondrup:okay

ADD REPLYlink written 8.7 years ago by Elena240
4
gravatar for Neilfws
8.7 years ago by
Neilfws48k
Sydney, Australia
Neilfws48k wrote:

There are lots of ways to address this but figuring out the start, stop and coding sequence from sequence alone is not the best of them.

Just go here, where you'll find that start codon = 139, last coding codon (before the stop) = 2469 and look at the CDS field for the protein sequence.

ADD COMMENTlink written 8.7 years ago by Neilfws48k
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