Question: Rna Data Sources???...Please Help
gravatar for Suhail
10.1 years ago by
Suhail10 wrote:

hi all,

I would like to have your comments on data source for RNA secondary structures at CRW site

I am not sure if the secondary structures presented on this site are determined experimentally (that means structures are proved to be the actual RNA structures) or if the structures are presented using some prediction methods (that means there is no proof that they are actual structures) any comment is appriciated…


rna secondary • 2.2k views
ADD COMMENTlink modified 9.4 years ago by Jarretinha3.3k • written 10.1 years ago by Suhail10
gravatar for Neilfws
10.1 years ago by
Sydney, Australia
Neilfws48k wrote:

The vast majority of RNA secondary structures are predicted, not determined experimentally. There are very many known RNAs and very few crystal (or other experimental) structures that contain RNA.

It is quite a confusing website, but there is a section called Methods: Structure Prediction with Comparative Sequence Analysis with more information about prediction methods.

ADD COMMENTlink written 10.1 years ago by Neilfws48k
gravatar for Jarretinha
10.1 years ago by
São Paulo, Brazil
Jarretinha3.3k wrote:

The known experimentally 2nd-ary structures are deposited in PDB (together with 3rd-ary :) ). Most came from NMR experiments.

Fortunatelly, in-silico predictors for RNA 2nd-ary structure are quite realiable and accurate. But, the on Guttel Lab the data are from comparative analysis, not from de novo predictions or experimentation. They rely on a specified known structural model. They're not all real structures, only models.

So, they're different from those on RFAM (the de facto standard). If you plan to explore completely new grounds, both information will be of some utility. By the way, RFAM has links to all known structures.

ADD COMMENTlink modified 4.3 years ago • written 10.1 years ago by Jarretinha3.3k

for filtered RNA and RNA-protein structures from PDB you can go here:

ADD REPLYlink written 4.3 years ago by lukaskoz20
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