Question: Map Smallrna-Seq On Mirna
0
gravatar for Nicolas Rosewick
5.7 years ago by
Belgium, Brussels
Nicolas Rosewick7.5k wrote:

Hi,

I've smallRNA-seq data and want to count the number of read for each miRNA. I'm studying samples from a non-well annotated species so I've to use mirbases sequences from a related species. My idea is to use bowtie to map the reads on miRNA mature sequences. But is bowtie a good idea to align small reads on small sequences ? Anyone has an another idea ?

Thanks

N.

mirna • 2.0k views
ADD COMMENTlink modified 5.7 years ago by Mike Axtell240 • written 5.7 years ago by Nicolas Rosewick7.5k
1
gravatar for Mike Axtell
5.7 years ago by
Mike Axtell240
Penn State University
Mike Axtell240 wrote:

See How can I accurately identify miRNA sequences from small RNA seq results

Short answer: For tallying exact matches to known mature miRNAs, a simple homemade string-matching script will suffice. But to also capture isomirs / processing variants / non-templated tailed species in your counts, an aligner is needed. Bowtie 1, Bowtie2, and SHRiMP2 have been reported to be fine.

ADD COMMENTlink modified 5.7 years ago by Istvan Albert ♦♦ 80k • written 5.7 years ago by Mike Axtell240

thanks. I didn't see the other post, sorry ;)

ADD REPLYlink written 5.7 years ago by Nicolas Rosewick7.5k
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