I followed the tutorial and setup local nr and nt databases and was able to get them working on the web interface, and was able to link them to my blast2go, and select the local server as a www-blast database.
A problem I am encounter however, is that no matter what I blast, i get no results returned. When I blast manually on the web server, I get a result, when I feed it to blast2go, and start the blast, everything gets no results returned.
This is somewhat odd. I even tried asking for my server for an Blast XML response, when I used the web server to search, and that worked just fine.
There is not much to go on, since there is no error anywhere... I was wondering if you had encountered this before? blast on NCBI right now works horrible for me, sometimes 5-10 minute per query... so I need to get the local one working asap.
My properties file for blast2go show: Blast.url=http://18.104.22.168/blast/blast.cgi Blast.urls=http://www.ncbi.nlm.nih.gov/blast/Blast.cgi,http://22.214.171.124/blast/blast.cgi Blast.blastdb=nt
Thank you for your time. Adrian
P.S. The URL is not accessible externally, only for people on intranet, but blast is run from a machine that has access to intranet. Hopefully that doesn't screw things up, there are no proxies setup.