Entering edit mode
10.2 years ago
ferris.us
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20
I have downloaded some SRA from NCBI which are SRR486147, SRR486148, SRR486149, SRR486150, SRR486151, SRR486214 and SRR486215. I also converted them to fastq files by using SRA Toolkit (fastq-dump --split-files SRR486147.sra). However the fastq files that were generated are not as usual. There are no sequences in the files. Are they correct? You can see a picture from the address below. Thanks!
Thanks for pointing it out! Do you know which tool is good for alignment? Can I use Tophat and bowtie2?
Tophat with bowtie1 should work.