How to solve multiple CDS features with same locus tag in EMBL format
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3.0 years ago

I have generated some EMBL files from gff3 format and am ending up with multiple CDS features at the same locus tag. From the ones I have checked, it is 2 CDS features per locus tag. Is this because there is one CDS on the forward strand and one on the reserves strand?

The tool I am wanting to use does not allow for multiple CDS on the same locus tag so how can I avoid this happening or a simple way to change it automatically?

Example EMBL: https://pastebin.com/LkzfB0nF

annotation embl • 1.3k views
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3.0 years ago
Juke34 8.5k

There are two CDS because there are two isoforms

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Is there a way I can avoid this? Or at least give different locus tags. I want to use antiSMASH and it does not allow multiple CDS at one locus tag

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Prior conversion you can use AGAT to keep only longest isoforms.

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This have solved the issue, thank you!

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