orthology enrichment

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2.9 years ago

isha.lily20 ▴ 10

Hello Researchers , 1) can any one tell me how to do a reciprocal blast ?

2)a) how to identify conserved orthologous genes and annotation of specific sequences ? and than 2)b) how to map specific sequences on conserved orthologous genes ?

i tried taking 3 protein sequences of different species and did normal protein blast with specific e value 1*10^(-6)

but i dont kno what is reciprocal blast , how to find evolutionary conserved orthologous genes ?

thnk u ....

orthology • 923 views

ADD COMMENT • link updated 2.8 years ago by Ram 43k • written 2.9 years ago by isha.lily20 ▴ 10

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If a Reciprocal Best Hit (RBH) search is what you want to do, try the easy-rbh module from MMseqs2.

As for your second (and maybe third) questions, dig around in this book perhaps?

ADD REPLY • link 2.9 years ago by Dunois ★ 2.5k

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Please stick to one question
Tell us what you've tried on your own.

ADD REPLY • link 2.9 years ago by Ram 43k

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Hi Ram, i tried the same as mentioned exported 3 protein sequences of different species just did normal protein blast

ADD REPLY • link 2.8 years ago by isha.lily20 ▴ 10

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Please google "reciprocal blast" - there are Biostars posts that address the topic. Always search online and try implementing a reliable solution you find on an online forum/paper.

ADD REPLY • link 2.8 years ago by Ram 43k

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