adding a contig fasta to reference genome fasta
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19 months ago
Elisa • 0

Hi everyone,

I'd like to add 'chr1_KI270763v1_alt.fa' (929.9 kB) sequence to my reference genome 'genome.fa' (grch38-3.2 GB). Is it possible and which tool shall I use ?

Thank you

reference genome • 490 views
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19 months ago
GenoMax 125k

Simply cat the two files together like so cat genome.fa alt.fa > new.fa (assuming they are both in fasta format).

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Remember that you'll need to regenerate new index files for this this new reference (.fai, .bwt, etc).

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