Remove PolyA 3'End with cutadapt
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11 months ago
GiV17 ▴ 40

Hi all, How Can I remove the polyA tails and the adapter sequences of my fastq file using cutadapt software? Has anyone tried it already? Thanks

sequencing • 569 views
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11 months ago
ATpoint 64k

Unsurprisingly the manual contains the answer in the FAQs:

https://cutadapt.readthedocs.io/en/stable/recipes.html#trim-poly-a-tails

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oh Yes, Thanks, But I would like a feedback with those who have already used it. And also I would like to understand which is the best polyA window to fling 10? 100?

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