I have Illumina sequencing results of a bacterial genome and a quality score of 35.89 is associated with these data. I know that a quality score of 30 is 99.99% of base calling accuracy based on this but what about the meaning of 35.89? Is there a scale used for quality score?
Thank you very much for your much appreciated help on this!
Can you elaborate a bit on how you obtained that quality score? Is that the average quality across all Illumina reads? Was it determined by a specific tool? If yes, how?
Thank you for your help. This quality score that I mentionned is the phred quality score. I would like to interpret this data properly.