How to perform multiple sequence alignment with large nucleotide sequence

Question

[Kumar]

Hi, I have a fasta file of 30 nucleotide sequences of the viral genome, and am looking to perform multiple sequence alignment on these sequences. Then I want to construct the phylogenetic tree between these sequences. I tried the MEGA X GUI version but it is unable to perform that large .fasta file. Please suggest any aligner that I can use and perform a phylogenetic tree at MEGA.

Thank you!

Answer 1

[GenoMax]

See: https://mafft.cbrc.jp/alignment/software/closelyrelatedviralgenomes.html