How to do transcriptome annotation through transcriptome annotation gtf file obtained after cuffllink ?

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2.1 years ago

isha.lily20 ▴ 10

Hello Researchers

Teacher gave work to do transcritpome annotation with only transcriptome annotation gtf file which was obtained after cufflink of 2 expression data control and test, the gtf file only contains exon.

how to find all annotation from these after cufflink gtf file

promoter/cds ?

work i tried already : from reference genome gtf file i seperated all annotated regions and extracted sequences than did blastn with transcriptome sequences and took top most hit, but teacher told focus on only transcriptome sequences for annotation, i am confuse plz help me.

Thank you

transcriptome annotation gtf • 456 views

ADD COMMENT • link 2.1 years ago by isha.lily20 ▴ 10

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The idea of assignments is to figure it out yourself. At least present some outline of ideas.

ADD REPLY • link 2.1 years ago by ATpoint 82k

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