different pileups between sorted.bam file and bigwig file
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2.0 years ago
bioyas ▴ 10

Hi,

I am using the following command from deeptools package to convert my sorted bam files to bigwig format to have smaller files that I can easily load into IGV.

bamCoverage --bam  Sample.sorted.bam -o  Sample.bw

I loaded both sorted.bam and bigwig file into IGV tool and I noticed that bigwig track doesn't show the pileups as they are in bam track.

Do you have any idea what I am doing anything wrong?

bigwig deeptools IGV • 621 views
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Details needed, such as a screenshot. We cannot see your screen or read minds.

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Hi, Here is two screenshots from random places. in each image the blue line is from bigwig file and below it is the sorted bam file.

enter image description here

enter image description here

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