Hi all,
I recently encountered a difficulty, there is a set of unstrand data through STAR or hisat2 to get bam fileand then through bedtools bamTobed into bed file, but I found in the bed file in the sixth column (strand column) appeared '+-', unstranded bed file of the sixth column should not be '.' Shouldn't it be .
This is the expected situation:
This is the observed situation:
Thanks in advance for the help!
Thank you very grateful for your reply. I also found this explanation in this site, but found 'no strand=.' on another site So should we follow that?Will the strand column of the bed file only show '+-' regardless of chain specificity?
Thank you very grateful for your reply. I'm sorry I got a concept wrong, I understand it now.