Entering edit mode
23 months ago
Candice
•
0
I am following the instruction to build the index using kb.
The command should be
kb ref -i index.idx -g t2g.txt -f1 cdna.fa -f2 intron.fa -c1 cdna_t2c.txt -c2 /
intron_t2c.txt --workflow lamanno -n 8 /
Mus_musculus.GRCm38.dna.primary_assembly.fa.gz /
Mus_musculus.GRCm38.98.gtf.gz
In Ensembl dog genome, there is no such file as dna.primary_assembly.fa.gz
. They're separate files (by chromosome) as shown here.
How could I create a dna.primary_assembly.fa.gz
for canine genome that includes all chromosomes?
Thanks!
You should be able to
cat
the files together.