Minimal insert size for Illumina MiniSeq
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23 months ago
MH85 ▴ 10

Hi everyone

What is the minimal insert size that can be reliably sequenced in Miniseq?

Is it okay to design a target DNA panel that has a region of interest (ROI) only 3bp long? Then, the insert size is going to be only between 50-70bp.

The intended application is target variant detection.

Thanks a lot in advance
Milos

insert-size MiniSeq amplicon Illumina • 375 views
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