TPM TMM DESeq2 normalization which choose to draw the heatmap for all samples

Question

[dew]

Dear guys,

May I know which method is recommended to draw the heatmap for all the samples(control, treat1, treat2, treat3... treat8)?

I read the articles/blogs and still do not fully understand the best choice...

Thank you very much for all your guidance!

Best,

Answer 1

[ATpoint]

You could follow this, using the normalized counts on log2 scale Scaling RNA-Seq data before clustering?