How can I generate the map.txt file require to detect novel miRNA using miReap
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21 months ago
khq5801 ▴ 10

I would like to detect novel miRNA from my miRNA NGS data. I will really appreciate if you would provide suggestions regarding generation of the map.txt file I can use to run the command in miReap. I have used the unmapped_file that I obtained from the alignment to miRBase mature miRNA database and aligned it to my reference genome using Bowtie. I am unable to generate the map.txt file similar to the sample map.txt file given in miReap software test directory. Kindly provide your valuable suggestion.

map.text miRNA miRDeep miReap • 574 views
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21 months ago
khq5801 ▴ 10

Found solution in this link....mireap procedure short tutorial

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