How to generate gene body coverage plot for tRNA?

Question

[PK]

Hi all,

I have bam files for WT and KO and it is a clip sequencing data. The reads are shorter in size so I hypothesised that my protein of interest preferentially binding to 3 primer end of the tRNA. So I would like to show as a something like gene body coverage for all tRNA population in human. But the problem is RSeQC requires BED12 format. Which I could not find. Is there any alternative to show that can represent the reads coverage for specific type of RNA. Is it possible to get BED12 format for tRNA(human)

Thanks