validation of pipeline for SV, SNV and repeat analysis
0
0
Entering edit mode
20 months ago
ashkan ▴ 160

I have built a pipeline for SV, SNV and repeat analysis using WGS data and I have used the pipeline for some of the datasets from our experiment that already I had. But I need to validate the pipeline. I have another pipeline for detecting variants including SNP and INDELs. To validate this pipeline I used vcfeval and as golden standard I used NA12878 dataset (GIAB). And then I will mostly look at sensitivity and precision. So the questions are, for the new pipeline which performs SV, SNV and repeat analysis:

1- can I used the same approach as variant calling pipeline?
2- if not, what tool can I use?
3- what data can I use as golden standard?
4- what criteria should be used to check the accuracy of the pipeline?
SV repeat SNV • 337 views
ADD COMMENT

Login before adding your answer.

Traffic: 1707 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6