How to find the read counts of sequences present in a file?
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8 weeks ago
khq5801 ▴ 10

I have annotated 500 miRNA sequences that range between 16-40 from blastout.fa file (original) using mature miRNA database and stored them in a file (myresults). Now, I would like to find the occurrence/read count of each sequence present in myresults file from blastout.fa file (original). I knew how to find the occurrence of a single sequence, but I am facing difficulties in finding the read counts of all the sequences present in a file (myresults) from blastout.fa (original). For instance, ACCGUACGACGACCGUACGACG is annotated and present in myresults file, I am able to find the readcount of this sequence in blastout.fa file, but I am facing difficulties in finding the readcounts of all the 500 sequences present in the file. Kindly suggest.

NGS blastn miRBase miRNA readcount • 261 views
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Please provide examples of the file content and format, besides how are you counting. It is hard to know what you have

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