Trimming sequencing primers from reads
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14 months ago
barzilayrom1 ▴ 10

Hi all,

I am going through a work-flow for analysis of paired-end reads data obtained from next-seq 500 (based on a published protocol) and it is noted that the sequencing primer should be trimmed from the reads. I wonder why should you do that, since as far as I understand the sequencing primer shouldn't even be a part of the read, or am I wrong?

I would really appreciate your feedback!

Thanks

trimming primers next-gen-sequencing • 725 views
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Sequencing primer should not be part of the reads. Perhaps the workflow meant to say adapters instead.

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Thanks! I thought so too, wonder though if it could happened by chance or due to error in base calling

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9 months ago
Fahad • 0

bash: java.jar/Trimmomatic-0.39/trimmomatic-0.39.jar: No such file or directory

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what is that ? is it a comment ? is it a question ?

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