I received a single cell data set from a biologist and would appreciate your assistance in understanding the experiment. To minimize costs, the cells from three samples of one condition were combined into a single pool, and a similar approach was taken for the second condition. Each condition consists of 12 fastq files, as depicted in the photo. Within one condition, there are three additional files located in the "filtered_feature_bc_matrix" folder: features.tsv.gz, barcode.tsv.gz, and matrix.mtx.gz.
May I inquire if it would be possible to perform a pseudo-bulk RNA-seq analysis to identify differential gene expression in this case? Is L001-L004 in each condition mean the lane and L001 is a sample? Your guidance and support would be greatly appreciated.