Highly Diffrentially Expressed miRNA from qPCR data
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14 months ago
Maria17 ▴ 40

Hello everyone, I working with miRNAs data and wants to apply diffrent methods in order to capture highly expressed miRNA and diffrentially expressed miRNAs.What are the available methods ?

Any recommendations or solutions would be appreciated.

Thanks Maria

miRNA R DEG • 452 views
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So you have qPCR data, right? Anything wrong with delta-delta CT as everyone does it?

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To compare the results, I would also like to try other methods. A delta ct value tells you which targets are highly expressed or low expressed, but I'm also interested in sub-groups of my targets. It is possible, for example, to cluster some targets together despite the fact that they are not highly or differentially expressed. It would be helpful if I could find methods for clustering and subgroups.

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