Entering edit mode
17 months ago
crx6xw
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10
Does cellranger scrnaseq normalize counts to transcript length?
Does cellranger scrnaseq normalize counts to transcript length?
Not that I am aware of. Unlike methods such as salmon it does genome alignment and does not resolve the exact transcript expression composition. Counts are just gene-level counts without modification (other thsn UMI deduplication).
As 10x Chromium is end-tagged anyway I am not convinced length correction would be robust or meaningful here.
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