What Pacbio HiFi data do people save ?

Question

[colindaven]

Hello all,

what PacBio data is worth saving long term?

• fastq
• bam (does this have additional signal for methylation calls etc )
• others ?

Thanks

Answer 1

[colindaven]

Ok, to answer my own question, bam is required for methylation analysis with the pacbio primrose software, so might be worth keeping too.

https://github.com/PacificBiosciences/primrose

[GenoMax]

If you have the means/budget then saving a copy of all raw data a sequencer produces (for some period of time) is likely a good policy, especially if you are a core and expect clients to ask for additional data. I assume you release fastq sequence as a minimal deliverable?

[colindaven]

That's good advice, if affordable. I just have little practical experience with Pacbio Hifi yet. In my core days we kept all data, even if there was never once a request for alternative demux from the bcl files.